The Tykes made sure there would be no giant-killing in front of the ITV cameras with a dominant first half display at the Camping World Community Stadium.
Nicky Cadden and John McAtee (2) put Barnsley 3-0 up inside 30 minutes, although the Hornets were much-improved in the second half.
Daniel Ajakaiye went close three times in the second period, but Horsham were unable to find a thoroughly-deserved consolation goal.
Here is a selection of excellent photos from the Cup clash courtesy of Natalie Mayhew, ButterflyFootie.
1. Horsham FC v Barnsley FC in the FA Cup: 26 brilliant pictures from the Hornets’ gutsy first round replay defeat to the Tykes
Action from Horsham v Barnsley Photo: Natalie Mayhew, ButterflyFootie
2. Horsham FC v Barnsley FC in the FA Cup: 26 brilliant pictures from the Hornets’ gutsy first round replay defeat to the Tykes
Action from Horsham v Barnsley Photo: Natalie Mayhew, ButterflyFootie
3. Horsham FC v Barnsley FC in the FA Cup: 26 brilliant pictures from the Hornets’ gutsy first round replay defeat to the Tykes
Action from Horsham v Barnsley Photo: Natalie Mayhew, ButterflyFootie
4. Horsham FC v Barnsley FC in the FA Cup: 26 brilliant pictures from the Hornets’ gutsy first round replay defeat to the Tykes
Action from Horsham v Barnsley Photo: Natalie Mayhew, ButterflyFootie